Prep HPLC Column, Bidentate C8, 4um, 100A. 30mm ID x 250mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑P30‑250 Cogent Bidentate C8 Preparative Column is designed for highest‑resolution preparative separations and demanding purification workflows, using 4 µm, 100 Å TYPE‑C™ silica hydride particles to deliver alternative selectivity, rapid equilibration, and exceptional durability.
The Bidentate C8 stationary phase provides moderate hydrophobic retention with distinct selectivity vs C18, making it ideal when:
C18 phases retain compounds too strongly
Improved recovery and faster elution are needed
Separating amine-containing or polar analytes
Processing high sample loads with fraction collection
With a 30 mm internal diameter, this column is fully in the preparative / purification range, supporting large injection volumes and maximum sample throughput.
Preparative Column Use‑Case Framework
250 mm Length – Maximum Separation & Peak Capacity
Highest resolving power in the entire 30 mm series
Ideal for very complex mixtures requiring maximum purity
Best suited for final purification or difficult separations
Longer run times and higher backpressure vs shorter columns
Flow Rate & System Requirements
30 mm ID preparative columns require specialized HPLC systems
Typical flow rate range: ~40 – 120 mL/min
Optimal starting range: ~35 – 60 mL/min for maximum resolution
Requires:
Preparative HPLC system with high‑flow capability
High-pressure, high-volume pump system
Fraction collection system
Reinforced tubing and fittings
⚠️ Standard analytical HPLC systems are not compatible with this column size.
Flow rate should be optimized based on:
Desired resolution vs throughput
Solvent viscosity
Sample loading
System pressure limits
Why Choose Bidentate C8 for Preparative Work
Compared to traditional C18 phases, Bidentate C8:
Reduces excessive retention of hydrophobic compounds
Improves recovery of strongly retained analytes
Provides alternative selectivity for polar and ionizable species
Enhances separation of basic compounds and amines
Improves throughput in preparative workflows
Cogent TYPE‑C™ Advantages
Silica Hydride Surface Technology
Reduced silanol interactions
Improved peak shape for basic compounds
Enhanced selectivity vs conventional silica
Faster Equilibration
Reduced cycle time between runs
Improved productivity in prep workflows
Exceptional Durability
Long column lifetime
Resistant to hydrolysis
Robust under high-load conditions
Typical Applications
Final purification of complex pharmaceutical mixtures
Isolation of metabolites and synthetic intermediates
Separation of highly retained compounds
Preparative chromatography requiring maximum resolution
Situations where C18 performance is limiting
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable